Electrophoresis I. Purpose ? perform ionophoresis using lying-in enzymes and lambda deoxyribonucleic acid ? understand how a barricade enzyme works ? analyse a photograph of cataphoresis ? understand how gel electrophoresis separates DNA molecules in a mixture ? how to use electrophoresis to separate DNA disassembles ? determine unknown DNA fragment sizes when given DNA fragments of known size II.
Materials agarose powder stamp tray and comb camera crushed ice container distilled water DNA samples electronic scale with tare electrophoresis box 250 ml Erlenmeyer flasks film graduated cylinder hood ice encumbrance dye microcentrifuge micropipet and tips 1.5 ml reaction tubes and racks restriction buffer restriction enzymes (HindIII, EcoRI, BamHI) 10x TEA buffer UV filter UV transilluminator 37? C water bath weighing boat III. operation Place the weighing boat on the scale and tare. deal out 0.8 ml of agarose powder and place it i...If you want to pee-pee a full essay, order it on our website: Ordercustompaper.com
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